Oxidation indicators comprising 3,3',5,5'-tetraalkylbenzidine compounds

US4385114A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Werner Guthlein · Mannheim, DE
• Walter Rittersdorf · Mannheim, DE
• Hugo Tiedemann
• Peter Vogel · Diepersdorf, DE
• Wolfgang Werner · Hersbruck, DE

Key dates

• Filing date: Feb 23, 1981
• Grant date: May 24, 1983
• Expiry date: Feb 23, 2001

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10T436/206664
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The detection and determination of (a) hydrogen peroxide or of a substance which reacts with the formation of hydrogen peroxide or (b) of peroxidase or a peroxidatively active substance, is carried out by contacting an unknown sample with a chromogen and, in case (a), with a peroxidatively active substance or in case (b) with hydrogen peroxide or a substance which reacts with the formation of hydrogen peroxide, and evaluating the coloration produced by the reaction, wherein, as the chromogen, there is used at least one 3,3',5,5'-tetraalkylbenzidine compound of the formula: ##STR1## wherein R.sub.1, R.sub.2, R.sub.3 and R.sub.4, which can be the same or different, are straight-chained or branched alkyl radicals containing up to 6 and preferably up to 4 carbon atoms.