Thermostable glycerokinases and process for its production
US4385120A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jul 16, 1981 |
| Grant date | May 24, 1983 |
| Priority date | — |
| Expiry date | Jul 16, 2001 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/832
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A thermostable glycerokinase enzyme useful in the detection and estimation of glycerol derivatives has a half-life in excess of 1 hour at 55.degree. C. at a protein concentration of less than 2 mg/ml and a pH of 7.8 .+-.0.5 in the absence of any substrate. The enzyme is produced by culturing at least one micro-organism which is capable of growth at a temperature of at least 50.degree. C. in a culture medium in which it will produce said enzyme, disrupting the resulting cells of the micro-organism to release the enzyme and separating the enzyme from the cell debris. The medium normally contains at least 0.1% glycerol but certain strains of micro-organism have been found to be capable of producing thermostable glycerokinase enzyme even in the absence of glycerol. The micro-organism is preferably a Bacillus organism, especially of the stearothermophilus species.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.