Immunoassays using F(AB').sub.2 fragments

US4397960A · kind A · utility

Assignee

• Technicon Instruments Corp. · US

Inventors

• Claude H. Moussebois · Brussels, BE
• Pierre L. Masson · Brussels, BE
• Jean-Pierre Vaerman · Wavre, BE
• Joseph Limet · Marchin, BE
• Cesar L. Cambiaso · Kraainem, BE

Key dates

• Filing date: Mar 26, 1982
• Grant date: Aug 9, 1983
• Expiry date: Mar 26, 2002

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/563
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Immunoassays for antigens or haptens are effected using, instead of an immunoglobulin antibody, the F(ab').sub.2 fragments thereof. In this way, interference from endogenous RF and Clq in the fluid under assay is avoided without the necessity of pre-treating the fluid to remove or inactivate the RF or Clq. The assays may be of various types including latex agglutination and competitive binding assays. A reagent for such use comprising F(ab').sub.2 fragments insolubilized on a water-insoluble substrate, especially a particulate substrate, is described.