Patent · US Expired

Method and reagent for the determination of glycerin

US4399218A · kind A · utility

30Cited by

6References

16Claims

0Family size

Assignee

Boehringer Mannheim GmbH · DE

Inventors

Helmgard Gauhl · Tutzing, DE
Hans Seidel · Tutzing, DE
Gunter Lang · Wernau, DE
Albert Roder · Seeshaupt, DE
Joachim Ziegenhorn · Starnberg, DE

Key dates

Filing date	Jan 23, 1981
Grant date	Aug 16, 1983
Priority date	—
Expiry date	Jan 23, 2001

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Q2326/96
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

For the determination of glycerin by oxidation with oxygen in the presence of glycerinoxidase and measurement of the oxygen consumption or of the H.sub.2 O.sub.2 formation, a glycerinoxidase from Aspergillus spec. DSM 1729 is used. A reagent suitable for this method consists of glycerinoxidase from Aspergillus spec. DSM 1729 and a system for the determination of H.sub.2 O.sub.2 and contains additionally, if desired, an agent for the saponification of esterified glycerin.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.