Enzyme production and purification
US4399221A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jun 26, 1981 |
| Grant date | Aug 16, 1983 |
| Priority date | — |
| Expiry date | Jun 26, 2001 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/818
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
There is described a process for the production of a polysaccharide-producing enzyme, which comprises fermenting an appropriate enzyme producing organism in aqueous sucrose, characterized in that the sucrose concentration is maintained at between 1 and 10 g/l throughout all but the last part of the fermentation and that the dissolved oxygen content of the fermentation is controlled. There is also described a method for the purification of a polysaccharide producing enzyme, which comprises absorbing a mixture containing the enzyme on an anionic resin and removing the enzyme from the resin with a sugar containing eluant, and a novel highly purified form of polysaccharide producing enzyme.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.