Process of using xanthomonas campestris NRRL B-12075 and NRRL B-12074 for making heteropolysaccharide
US4400467A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Apr 7, 1981 |
| Grant date | Aug 23, 1983 |
| Priority date | — |
| Expiry date | Apr 7, 2001 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/91
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This invention involves the production of heteropolysaccharides such as xanthan gum by the continuous fermentation of suitable nutrient media with new degenerative resistant variants of Xanthomanas campestris. Fermentation is conducted in a nitrogen, sulfur or phosphorous limited nutrient medium containing glucose or a minimal medium plus yeast extract and glutamic acid, in a first fermenter to enhance cultural growth. After the limiting growth element (nitrogen, sulfur or phosphorous) has been essentially consumed, the medium is then transferred to a second fermenter and fermentation continued while adding a suitable carbohydrate which the bacteria convert into the heteropolysaccharide product. The process may be modified by carrying out cultural growth and production of the polysaccharide in the same fermenter. The bacteria employed were cultured from a glucose-mineral salts medium fortified with yeast extract or a glucose-defined minimal medium in which ammonium chloride served as the sole assimilable nitrogen source.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.