Detection and isolation of glucagon mRNA using a synthetic oligodeoxynucleotide
US4401759A · kind A · utility
Inventors
Key dates
| Filing date | Apr 8, 1982 |
| Grant date | Aug 30, 1983 |
| Priority date | — |
| Expiry date | Apr 8, 2002 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/143333
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A synthetic oligodeoxynucleotide complementary to glucagon mRNA and a method of using it to detect and isolate glucagon mRNA and cDNA from human and rabbit pancreas. A unique 14 base oligodeoxynucleotide dTTCATCAGCCACTG wherein T represents thymine, G represents guanine, A represents adenine, and C represents cytosine and where at least 13 of the 14 nucleotides are as indicated (one of those indicated may be replaced by one of the other three mentioned nucleotides) has been found to be complementary to glucagon mRNA. To isolate glucagon mRNA, total RNA is first extracted from human or rabbit pancreas and A+ RNA (mRNA-poly A) is isolated from the total RNA. The A+ RNA is then treated with the oligodeoxynucleotide, and the resulting hybridized RNA is enzymatically converted to glucagon mRNA which can then be purified, copied into glucagon cDNA and used in a conventional manner to produce glucagon by cloning technique.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.