Immunoassays employing labeled reagent and a conjugate having two binding sites
US4401764A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Feb 2, 1981 |
| Grant date | Aug 30, 1983 |
| Priority date | — |
| Expiry date | Feb 2, 2001 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/828
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
In the immunoassay of antigens in liquids, a reaction mixture is formed containing the liquid under assay, labelled antigen, and a mixed binding reagent which contains an antigen-binding site and a label-binding site, the two sites being spaced apart in the reagent so that a single molecule of labelled antigen cannot bind to both sites. The label is one whose activity is changed upon binding to a label-binding site, and the amount of antigen in the original liquid sample is determined by measuring the activity of the label in the reaction mixture. A preferred label is a fluorophore. The mixed binding reagent preferably consists of two antibodies linked together.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.