Continuous spectrophotometric assay of microbial cellulase
US4403032A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jan 25, 1982 |
| Grant date | Sep 6, 1983 |
| Priority date | — |
| Expiry date | Jan 25, 2002 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/808
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A continuous spectrophotometric assay monitors the release of colored cellulose oligosaccharides during the cellulase catalyzed hydrolysis of dyed crystalline cellulose preparations. An anthraquinone derivative, Remazol Brilliant Blue R-Salt, is used to uniformly dye a variety of cellulose biopolymers without altering their respective polysaccharide conformations. The increase in dye absorbance of a cellulose filtrate is a direct measure of the total concentration of carbohydrates formed and the cellulase activity. The continuous cellulase assay has specific utility in the study of cellulase kinetics and in comparing the cellulase activities of different microorganisms.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.