Method for quantitatively determining a particular substrate catalyzed by a multisubstrate enzyme
US4404066A · kind A · utility
448Cited by
6References
9Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | May 13, 1982 |
| Grant date | Sep 13, 1983 |
| Priority date | — |
| Expiry date | May 13, 2002 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/817
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for control of relative enzyme activity for analytical purposes. The activity of a multisubstrate enzyme may be controlled as a function of the electrical potential applied to the enzyme. The enzyme is preferrably incorporated into a thin layer electrochemical cell laminate having exterior membrane layers and an interior enzyme layer. A control electrode located within the enzyme layer applies an electrical potential to the enzyme. An intermediate electron transfer agent may be used to transfer electrons to and from the enzyme and the control electrode.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.