Methods and compositions for assaying for the production of 1,4-dihydropyridyl
US4404278A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Nov 24, 1980 |
| Grant date | Sep 13, 1983 |
| Priority date | — |
| Expiry date | Nov 24, 2000 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/808
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Methods and compositions for assaying for coenzymes having N-substituted 1,4-dihydropyridyl as the active portion of the prosthetic group. The conversion of NAD to NADH is widely measured both for determining NAD-NADH (including the analogs thereof) dependent enzymes and enzymes that can be coupled to NAD-NADH dependent enzymes, and for determining ligands or receptors in competitive protein binding assays. Antibodies specific for NADH in the presence of NAD are employed, providing enhanced fluorescence over the normal fluorescence of unbound NADH. By determining the rate of formation of NADH or the concentration of NADH, the analyte or enzyme of interest can be determined.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.