Bilirubin assay
US4404286A · kind A · utility
9Cited by
6References
12Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Feb 22, 1982 |
| Grant date | Sep 13, 1983 |
| Priority date | — |
| Expiry date | Feb 22, 2002 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/25125
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An improved reagent and method for assay of total bilirubin in biological fluids utilizes diazo sulfanilic acid to convert bilirubin to azobilirubin which is then quantitatively measured. The improved reagent and method include the solvent accelerator tetramethylene sulfone. The use of tetramethylene sulfone as also allows the use of nonionic surfactants, obviating the need for serum blank correction in highly lipemic serum. Furthermore, the use of tetramethylene sulfone and nonionic surfactants renders the reagent mixture less viscous facilitating automated analysis.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.