Beta-galactosyl-umbelliferone-labeled hapten conjugates
US4404366A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jul 16, 1981 |
| Grant date | Sep 13, 1983 |
| Priority date | — |
| Expiry date | Jul 16, 2001 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/535
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An improved specific binding assay method and reagent for determining a ligand in a liquid medium employing, as an enzyme-cleavable substrate label, a residue having the formula: EQU G--D--R wherein G is a glycone, D is a dye indicator moiety, and R is a linking group through which the label residue is covalently bound to a binding component of a conventional binding assay system, such as the ligand, an analog thereof, or a specific binding partner thereof. The monitored characteristic of the label is the release of a detectable product, usually a fluorogen or chromogen, upon enzymatic cleavage of the glycosidic linkage between the glycone and the dye indicator moiety. The assay method may follow a homogeneous or heterogeneous format. The preferred glycone is a .beta.-galactosyl group and the preferred dye indicator moiety is an umbelliferone residue. The improved assay is particularly suited to the determination of haptens, such as drugs, and antigenic proteins and polypeptides, including antibodies, following a homogeneous competitive binding assay format. PAC CROSS-REFERENCE TO RELATED APPLICATION
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.