Process for obtaining nerve growth factor
US4407744A · kind A · utility
Inventor
Key dates
| Filing date | Feb 5, 1979 |
| Grant date | Oct 4, 1983 |
| Priority date | — |
| Expiry date | Feb 5, 1999 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/854
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A new form of nerve growth factor having a molecular weight of about 116,000 and which is stable in dilute aqueous solutions is obtained from mouse submandibular gland or mouse saliva. An extract of mouse submandibular gland or mouse saliva is subjected to ion-exchange chromatography to recover a component of the gland or saliva rich in the nerve growth factor and containing proteinaceous material which does not degrade the nerve growth factor. The pure nerve growth factor is obtained by subjecting the component rich in nerve growth factor to at least one additional chromatography step, usually two to four, to recover pure nerve growth factor having a molecular weight of about 116,000.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.