Immobilized antibody or antigen for immunoassay
US4407943A · kind A · utility
162Cited by
9References
6Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Mar 13, 1980 |
| Grant date | Oct 4, 1983 |
| Priority date | — |
| Expiry date | Mar 13, 2000 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/811
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An antigen or antibody and an immunochemically neutral protein are immobilized in two stages on a microporous membrane coated with a protein such as zein or collagen. Immobilized antigen to Toxoplasma gondii is used in an immunoassay to detect the presence of antibody to Toxoplasma gondii in serum. Antibody that becomes bound to the immobilized antigen is detected with an antibody- enzyme conjugate. Immobilizing the immunochemically netural protein on the membrane in a second stage immobilization step minimizes nonspecific binding during the immunoassay.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.