Process for obtaining metal-free corrinoids

US4407947A · kind A · utility

Assignee

• Gesellschaft fur Biotechnologische Forschung mbH (GBF) · DE

Inventors

• Volker Koppenhagen · Braunschweig, DE
• Gerhard Schlingmann · Texico, US
• Bernd Dresow · Braunschweig, DE
• Ludwig Bischoff · Wolfenbüttel, DE
• Rosemarie Penkert · Braunschweig, DE
• Eicke Siefert · Göttingen, DE

Key dates

• Filing date: Sep 14, 1981
• Grant date: Oct 4, 1983
• Expiry date: Sep 14, 2001

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12P17/182
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A process for obtaining metal-free corrinoids consisting of inoculating a culture media free of added compounds of metals of the VIIIth group of the Periodic Table with a parent strain of Rhodopseudomonas, cultivating under illumination, repeating the steps of inoculating and cultivating employing said culture media and said illumination employing a part of the sequential culture from the preceding stage as an inoculum in each subsequent state, for a series of several pre-production culture stages, inoculating said culture media with the preceding sequential culture for the last time, cultivating under illumination, separating the microorganism from the culture media of the production culture stage and isolating metal-free corrinoids from the culture media of the production culture stage.