Process and reagent for the determination of the biological activity of heparin in plasma

US4409327A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Knut Bartl · Weilheim in Oberbayern, DE
• Helmut Lill · Wielenbach, DE
• Peter Roeschlau · Seeshaupt, DE
• Joachim Ziegenhorn · Starnberg, DE

Key dates

• Filing date: Feb 11, 1981
• Grant date: Oct 11, 1983
• Expiry date: Feb 11, 2001

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S435/81
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Method and reagent for the determination of the biological activity of heparin in plasma by adding thrombin or Factor X.sub.a as the proteolytic enzyme and a chromogen substrate of the latter and measuring the dye liberated from the chromogen substrate in the absence of exogenous anti-thrombin III, the improvement consisting of adding, additionally, at least one compound of the formula ##STR1## wherein X is oxygen or the group NR.sub.5 ; PA1 and R.sub.1, R.sub.2, R.sub.3, R.sub.4 and R.sub.5 are independently selected from hydrogen or alkyl of 1 to 2 carbon atoms.