Method and reagent for the determination of glycerol

US4409328A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Joachim Ziegenhorn · Starnberg, DE
• Knut Bartl · Weilheim in Oberbayern, DE
• Albert Roder · Seeshaupt, DE
• Gunter Lang · Wernau, DE
• Hans Mollering · Tutzing, DE
• Ulrich Nagele · Bernried, DE

Key dates

• Filing date: Sep 16, 1981
• Grant date: Oct 11, 1983
• Expiry date: Sep 16, 2001

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S435/81
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

For the determination of glycerol, the latter is incubated with galactose oxidase in an aqueous medium in the presence of oxygen and either the oxygen consumption or the amount of H.sub.2 O.sub.2 or glyceraldehyde that is formed is determined. A reagent suitable for this purpose consists of galactose oxidase and a system for the determination of H.sub.2 O.sub.2 or a system for the determination of glyceraldehyde. It can additionally contain an agent for the saponification of esterified glycerol.