Method for eliminating glucose dependent Schiff base effect from hemoglobin A.sub.1 assay
US4409335A · kind A · utility
33Cited by
8References
16Claims
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Key dates
| Filing date | May 28, 1982 |
| Grant date | Oct 11, 1983 |
| Priority date | — |
| Expiry date | May 28, 2002 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/144444
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An ion exchange method for the separation of hemoglobin A.sub.1 from its Schiff base precursors and from nonglycosylated hemoglobin in a sample of human blood. The known method of lysing the sample, using it to impregnate a weak cation exchange resin, eluting out the glycosylated components with a buffer solution containing from about 0.6 M to about 0.11 M alkali metal ion dissolved therein, and recovering the eluate, is modified by the inclusion of a dihydroxyboryl compound in either the hemolysate, the elution buffer, or both.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.