Endoproteinase-Lys-C and process for its preparation thereof
US4414332A · kind A · utility
1Cited by
0References
5Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Aug 28, 1981 |
| Grant date | Nov 8, 1983 |
| Priority date | — |
| Expiry date | Aug 28, 2001 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/822
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides endoproteinase-Lys-C from bacteria, consisting of a chain of molecular weight 35000 to 38000 Dalton, having a pH optimum at pH 7.7 and being inhibited by aprotinin but not being inhibited by alpha.sub.2 -macroglobulin, .alpha..sub.1 -antitrypsin and ethylenediamine-tetraacetic acid. The present invention also provides a process for obtaining this enzyme, wherein a culture broth of an appropriate bacterial strain which has been filtered or pre-purified by usual methods is chromatographed over carrier-fixed alpha.sub.2 -macroglobulin-metal complex and the enzyme is obtained from the filtrate.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.