Process for producing an insulin
US4421685A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | May 11, 1981 |
| Grant date | Dec 20, 1983 |
| Priority date | — |
| Expiry date | May 11, 2001 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K1/067
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Production of insulin or an insulin analog is provided by combination of an A-chain and a B-chain, which comprises bringing the S-sulfonated form of the A-chain, the S-sulfonated form of the B-chain, and a thiol reducing agent together in an aqueous medium under conditions which produce a mixture having (1) a pH of from about 8 to about 12, (2) a total protein concentration of from about 0.1 to about 50 milligrams per milliliter, and (3) an amount of thiol reducing agent which affords a total of from about 0.4 to about 2.5 --SH groups per each --SSO.sub.3.sup.- group present in the total amount of A- and B-chain S-sulfonates, and allowing formation of insulin or an insulin analog to occur by maintaining the mixture at a temperature of from about 0.degree. C. to about 25.degree. C. and in an environment which provides a source of oxygen.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.