Immobilization of enzymes
US4421850A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Feb 24, 1981 |
| Grant date | Dec 20, 1983 |
| Priority date | — |
| Expiry date | Feb 24, 2001 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N11/14
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
An immobilized enzyme product is produced by contacting an inert particulate support with an aqueous enzyme solution containing preferably at least 25% dissolved solids and with a water miscible organic solvent, and crosslinking the enzyme to insolubilize the enzyme on the support as a gel containing 50-90% water. The water miscible organic solvent is in substantial excess of the amount of water mixed with the support in the process. The solvent may be contacted with the support before or after contacting with the enzyme solution. The resultant immobilized enzyme product has a bulk volume 5 to 300% greater than the bulk volume of the support material. A least a portion of the gel is external to the support and constitutes at least 3% by volume of the immobilized enzyme product.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.