Centrifuge rotor apparatus for preparing particle spreads

US4423699A · kind A · utility

Assignee

• E.I. DU PONT DE NEMOURS AND COMPANY · US

Inventors

• John W. Boeckel · Southbury, US
• Vernon C. Rohde · Newtown, US
• John R. Wells · Culver City, US

Key dates

• Filing date: Feb 28, 1979
• Grant date: Jan 3, 1984
• Expiry date: Feb 28, 1999

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2001/2846
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A centrifuge rotor is described which facilitates the preparation of cell dispersions on microscope slides. The rotor is bowl-like in configuration and defines an annular channel having plural circumferentially disposed septa. The septa provide plural separate regions each adapted to receive removable chambers. The outer wall in each region is flat to accommodate a microscope slide. Each chamber has an outlet orifice adapted to contact the microscope slide. A sample containing blood cells, for example, may be placed in each chamber and the cells centrifugally sedimented against the slide associated with the respective chambers. A gasket at the interface between each slide and its chamber prevents leakage of the sample and a conduit in each chamber permits removal of the supernatant fluid following cell sedimentation.