Particle agglutination immunoassay with agglutinator for determining haptens; PACIA

US4427781A · kind A · utility

Assignee

• International Institute of Cellular and Molecular Pathology · BE

Inventors

• Pierre L. Masson · Brussels, BE
• Daniel Collet-Cassart · Limal, BE
• Carl-Gustaf M. Magnusson · Brussels, BE

Key dates

• Filing date: Jan 24, 1984
• Grant date: Jan 24, 1984
• Expiry date: Jan 24, 2004

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S436/825
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A particle counting assay for haptens (small non-protein monovalent substances having a molecular weight below 1500) comprises mixing a liquid sample (e.g. of human origin) containing the hapten, with finely divided inert particles bearing the same hapten (or a specific analogue thereof), an agglutinator such as RF or C1q, and a measured amount of antibody, which amount is insufficient to cause agglutination of all the particles. The amount of hapten is determinable by measuring the extent of the agglutination.