Affinity chromatography using metal ions
US4431546A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Apr 26, 1982 |
| Grant date | Feb 14, 1984 |
| Priority date | — |
| Expiry date | Apr 26, 2002 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01J20/3255
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
A process for the affinity chromatographic separation of at least one biological or related substance from a mixture wherein the at least one biological or related substance is bound to a binding material, having a ligand containing at least one of the groups anthraquinone, phthalocyanine or aromatic azo, in the presence of at least one metal ion selected from the group Ca.sup.2+, Sr.sup.2+, Ba.sup.2+, Al.sup.3+, Co.sup.2+, Ni.sup.2+, Cu.sup.2+ or Zn.sup.2+. The preferred metal ions are Co.sup.2+, Ni.sup.2+ and Zn.sup.2+, with Zn.sup.2+ being particularly preferred. The ligand may be linked directly to the matrix or via a spacer arm. The process may be performed at atmospheric pressure or under pressure, especially high pressure (100-3500 psi). The nature of the contact, washing and eluting solutions depends on the substance to be separated. Generally the contact solution is made up of the substance to be separated and a metal salt dissolved in a buffer solution, while the washing solution comprises the same metal salt dissolved in the same buffer. The eluting solution, may be a buffer solution, either alone or containing a chelating agent or it may be an alkali metal salt or a spe…
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