Process for obtaining hepatitis-safe, sterile hemoglobin solutions free of pyrogens and stroma

US4439357A · kind A · utility

Assignee

• Biotest-Serum-Institut GmbH

Inventors

• Klaus Bonhard · Hanau, DE
• Bertram Eichentopf · Bad Soden, DE
• Norbert Kothe · Kronberg, DE

Key dates

• Filing date: Jul 30, 1982
• Grant date: Mar 27, 1984
• Expiry date: Jul 30, 2002

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S530/829
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

A process for obtaining hepatitis-safe, sterile, pyrogen- and stroma-free hemoglobin solutions of low potassium content comprising stirring an erythrocyte concentrate with about 1 to 6-times its volume of an about 5 to 15% aqueous solution of a sugar, sugar alcohol or high-molecular-weight colloid, adjusting the pH of the resulting suspension to about 5 to 6.5 with an acid which is physiologically tolerable as salt, allowing the erythrocytes to settle, removing the supernatant from the sediments, adjusting the pH of the sediment to about 7 to 8, homogenizing the sediment, adjusting its hematocrit to about 55 to 60%, treating the mass with a dilute beta-propiolactone solution, hemolyzing the residual solids and forming a suspension thereof, contacting the suspension with a cation exchanger in H.sup.+ form until the pH has decreased to about 5 to 5.5 thereby to precipitate the stroma, separating from the liquid the cation exchanger and precipitated stroma, and adjusting the pH to about 7.2 to 7.6.