Tripeptide derivatives and their application in assaying enzymes
US4440678A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Sep 16, 1981 |
| Grant date | Apr 3, 1984 |
| Priority date | — |
| Expiry date | Sep 16, 2001 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/109163
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Tripeptide derivatives having the formula ##STR1## wherein R.sup.1 is alkanoyl or .omega.-aminoalkyanoyl, phenylalkanoyl or p-aminophenylalkanoyl, cyclohexylcarbonyl or 4-aminomethyl-cylcohexylcarbonyl, benzoyl optionally substituted with methyl, amino, or halogen in the o- or p-position, alkoxy carbonyl, benzyloxy carbonyl optionally substituted with methoxy, methyl, or chlorine in the p-position, alkylsulfonyl, phenylsulfonyl or naphthylsulfonyl, R.sup.2 is straight-chained or branched alkyl, hydroxyalkyl, alkoxyalkyl, benzoxyalkyl, .omega.-carboxy-alkyl, .omega.-alkoxy carbonylalkyl, .omega.-benzyloxycarbonylalkyl, cyclohexyl, cyclohexylmethyl, 4-hydroxycyclohexylmethyl, phenyl, benzyl, 4-hydroxybenzyl or imidazol-4-yl-methyl, R.sup.3 is hydrogen or alkyl, R.sup.4 is hydrogen, methyl or ethyl, and R.sub.5 is an amino group substituted with aromatic or heterocyclic radicals, R.sup.5 being capable of being split off by enzymatic hydrolysis to form a colored or fluorescent product H-R.sup.5. The tripeptide derivatives of formula I are used for assaying certain enzymes, and in particular, factor Xa. Enzyme-bearing materials are reacted with the said tripeptide derivatives. The quant…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.