Method for producing recombinant bacterial plasmids containing the coding sequences of higher organisms

US4440859A · kind A · utility

Assignee

• The Regents of the University of California · US

Inventors

• William J. Rutter · San Francisco, US
• Howard Goodman · Newtown, US
• Axel Ullrich · Portola Valley, US
• John Shine · Fawn Drive, AU
• John Chirgwin · San Francisco, US
• Raymond Pictet · San Francisco, US

Key dates

• Filing date: Sep 11, 1981
• Grant date: Apr 3, 1984
• Expiry date: Sep 11, 2001

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/00
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Microorganism having a gene derived from a higher organism is produced by isolating cells from a higher organism containing messenger RNA, extracting the messenger RNA, synthesizing a double stranded cDNA using the messenger RNA as a template, inserting the cDNA into a plasmid and transforming a microorganism with the resultant recombinant plasmid.