Azo dyestuff for use as a chromogen in detecting leukocytes

US4442033A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Dieter Berger · Viernheim, DE
• Franz-Josef Braun · Rimbach, DE
• Gunter Frey · Ellerstadt, DE
• Werner Guthlein · Mannheim, DE
• Manfred Kuhr

Key dates

• Filing date: Jun 8, 1981
• Grant date: Apr 10, 1984
• Expiry date: Jun 8, 2001

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/5094
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Azo dyestuff ester of the formula EQU A--N.dbd.N--B(OR).sub.n wherein PA0 A is a five- or six-membered heterocyclic radical of up to 2 hetero ring atoms selected from nitrogen, sulfur and oxygen, and wherein the heterocyclic ring may be substituted with at least one member of the group selected from halogen, lower alkyl and lower alkoxy; or PA0 A is phenyl substituted by at least one member of the group selected from lower alkyl, lower alkoxy, nitro, sulphanato and acylamino; PA0 B is benzene, naphthalene or quinoline, optionally substituted with one or two radicals selected from sulphanato, lower alkoxy and lower alkoxypoly-alkyleneoxy; PA0 R is a carboxylic acid residue or an amino acid or peptide residue having a nitrogen protective group; and PA0 n is 1 or 2. This dyestuff is particularly useful as a chromogen in a diagnostic composition for detecting leukocytes in body fluids.