Patent · US Expired

Process for preparing L-.alpha.-methylphenyl alanines

US4443548A · kind A · utility

10Cited by
2References
5Claims
0Family size

Assignee

Inventors

Key dates

Filing dateMay 7, 1982
Grant dateApr 17, 1984
Priority date
Expiry dateMay 7, 2002

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12N9/80
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

Biochemical optical resolution of DL-.alpha.-methylphenyl alanines in which DL-.alpha.-methylphenyl alanine amides are interacted with the culture products, or their treated products, of a microorganism capable of producing amidase is described. L-.alpha.-methylphenyl alanines having the general formula (I): ##STR1## wherein R.sub.1 and R.sub.2 may be independently a hydrogen atom or lower alkyl groups, or R.sub.1 and R.sub.2 may be alkylene groups combined together to form 5 through 8-membered rings is produced by the steps of: PA1 (a) making a DL-.alpha.-methylphenyl alanine amide having the general formula (II): ##STR2## wherein R.sub.1 and R.sub.2 are the same as defined above, interact with the culture product of a microorganism capable of producing enzyme catalyzing the hydrolysis of L-isomer of DL-.alpha.-methylphenyl alanine amides or the treated product thereof, whereby asymmetric hydrolysis of an L-.alpha.-methylphenyl alanine amide is effected; and PA1 (b) separating the resultant L-.alpha.-methylphenyl alanines from the hydrolysis mixture.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.