Glass-surface microcarrier for growth of cell cultures
US4448884A · kind A · utility
21Cited by
5References
7Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Mar 3, 1982 |
| Grant date | May 15, 1984 |
| Priority date | — |
| Expiry date | Mar 3, 2002 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2533/30
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A microcarrier for anchorage-dependent cell cultivation is prepared containing a spherical substrate of polymeric material having a bulk density of about 1 g/cc so as to be substantially buoyant in an aqueous culture medium, and a thin (less than 1 .mu.m) coating layer of silicate glass. The silicate glass coating layer is preferably applied to a spherical precursor of polymeric material in an rf sputtering operation. An intermediate coating layer of magnetic material may be deposited prior to the silicate glass layer, so that the microcarriers may be readily removed from culture media by suitable subjection to a magnetic field.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.