Process for recovering human IFN-.beta. from a transformed microorganism
US4450103A · kind A · utility
59Cited by
1References
29Claims
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Key dates
| Filing date | Mar 1, 1982 |
| Grant date | May 22, 1984 |
| Priority date | — |
| Expiry date | Mar 1, 2002 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K14/565
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A process for recovering IFN-.beta. from transformed bacteria comprising: disrupting the cell membranes of the bacteria; solubilizing the IFN-.beta. from the disruptate into an aqueous medium with a solubilizing agent such as sodium dodecyl sulfate; extracting the IFN-.beta. from the aqueous medium with 2-butanol, 2-methyl-butanol, or mixtures thereof under conditions that maintain phase separation between the aqueous meduim and the extractant; and isolating the IFN-.beta. from the extractant such as by precipitating the IFN-.beta. from an aqeous buffer mixture of the extractant by lowering the pH thereof.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.