Incorporation of pyridoxal phosphate in dry analytical elements for the determination of enzymes
US4450232A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Feb 4, 1982 |
| Grant date | May 22, 1984 |
| Priority date | — |
| Expiry date | Feb 4, 2002 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/805
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Enzymes which are activated by pyridoxal phosphate are assayed in a dry analytical element which includes a high coverage of pyridoxal phosphate. The coverage of pyridoxal phosphate is such that the multiplication product of the coverage and the spreading coefficient of the element results in a reagent mixture concentration of at least about 0.9 mmole per liter when a liquid sample is contacted with the element thereby eliminating the need for a preincubation of the sample with a solution containing pyridoxal phosphate. The reactions for the determination of the enzymes are initiated by contact of the dry elements with a liquid sample.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.