Immunoassay of proteins

US4455381A · kind A · utility

Assignee

• International Institute of Cellular and Molecular Pathology · BE

Inventors

• Carl-Gustaf M. Magnusson · Brussels, BE
• Daniel Collet-Cassart · Limal, BE
• Pierre L. Masson · Brussels, BE

Key dates

• Filing date: Nov 6, 1981
• Grant date: Jun 19, 1984
• Expiry date: Nov 6, 2001

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S436/825
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

In the immunoassay of a particular protein in a biological fluid, there is frequently interference in the assay by other proteins present in the fluid, e.g. by complement factors or antibodies in human serum. The interference so caused can be avoided by subjecting the fluid to protein-digestion, using for example an enzyme such as pepsin, as a result of which the particular protein of interest, or a fragment thereof, can be assayed without interference by the other proteins. Also, radioallergosorbent tests for particular IgE antibodies can be improved in sensitivity and accuracy, by subjecting the absorbed IgE to enzymic digestion, and then assaying a fragment thereof.