Patent · US Expired

Device and method for controlled freezing of cell cultures

US4455842A · kind A · utility

35Cited by
15References
6Claims
0Family size

Assignee

Inventor

Key dates

Filing dateSep 27, 1982
Grant dateJun 26, 1984
Priority date
Expiry dateSep 27, 2002

Classification

  • Technology area (CPC F)Mechanical Engineering; Lighting; Heating
  • CPC primaryF25D9/00
  • WIPO fieldBasic materials chemistry
  • WIPO sectorChemistry

Abstract

An enclosed device and method for controlled freezing of cell cultures, especially eucaryotic cell cultures, contained in cell culture vials by means of a conventional freezer includes a container and top, a supporting grid and supporting grid base within the container for supporting the culture vials and a compatible liquid refrigerant in a volume of at least fifteen times the culture volume and at a level to totally immerse all of the cultures to be frozen. Compatible liquid refrigerants include the straight chain aliphatic alcohols, preferrably ethanol and methanol.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.