Sensitive immunoassays of antigens or antibodies sequestered within immune complexes
US4459359A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Nov 19, 1981 |
| Grant date | Jul 10, 1984 |
| Priority date | — |
| Expiry date | Nov 19, 2001 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/828
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A process for determining the presence of an antigen or antibody in a sample wherein said antigen or antibody exists in the form of an immune complex which comprises: PA0 A. contacting the immune complex originating from the sample suspected of containing immune complex with a dissociating buffer whereby said immune complex, if present, is dissociated into antigen and antibody; PA0 B. contacting a solid support which binds proteins with said dissociating buffer suspected of containing antigen or antibody and removing said buffer; PA0 C. washing said solid support; PA0 D. adding protein to fill unoccupied sites on said solid support; PA0 E. adding radioactively labeled or enzyme labeled antibody or antigen to said solid support, said labeled antibody or antigen corresponding to antigen or antibody on said solid support, incubating the resultant mass and washing the same; PA0 F. measuring the radioactivity or enzymatic activity associated with the solid support.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.