Cell-driven viral transfer in eukaryotes
US4464465A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Apr 5, 1982 |
| Grant date | Aug 7, 1984 |
| Priority date | — |
| Expiry date | Apr 5, 2002 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/948
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Novel method, cells and compositions are provided involving transforming B-lymphocytes to provide immortalization for continuous production of monoclonal antibodies to a predetermined ligand. T-cell free B-lymphocytes are combined with an Epstein-Barr virus transformed cell sensitive to a cytotoxic agent which does not significantly affect the B-lymphocytes under conditions where the sensitive EBV transformed cell acting as the transfer agent is killed and efficiently transforms the B-lymphocyte recipient cells with EBV. The EBV transformed B-lymphocyte cells are amplified and cloned, the desired clones isolated in accordance with conventional techniques and then used for production of monoclonal antibodies.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.