Identification and enumeration of microbial cells
US4467032A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Apr 26, 1982 |
| Grant date | Aug 21, 1984 |
| Priority date | — |
| Expiry date | Apr 26, 2002 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N21/23
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A process and apparatus is disclosed for rapid and accurate identifying and enumerating of microorganisms. A sample believed to contain a certain microorganism is combined with an inhibiting reagent having a known specificity for the microorganism. The combined sample and inhibiting reagent is placed in an alternating current electrical field having a frequency sufficiently high to cause alignment of microbial cells in the sample. A polarized beam of laser light is then passed through the sample and the extent of birefringence is measured. The measured birefringence is compared with that of a control sample known to contain the microorganism under investigation to determine the microorganism in the sample.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.