Purification of 12-O-tetradecanoylphorbol-13-acetate, phorbol, and 4.alpha. -
US4468328A · kind A · utility
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9Claims
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Key dates
| Filing date | Jan 17, 1983 |
| Grant date | Aug 28, 1984 |
| Priority date | — |
| Expiry date | Jan 17, 2003 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07C69/013
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Droplet counter-current chromatography is utilized to isolate and purify 12-O-tetradecanoylphorbol-13-acetate (TPA), phorbol, and the diastereoisomer 4.alpha.-phorbol from croton oil. The stationary and mobile phases for the procedure are derived from an equilibrated mixture of hexane-diethylether-n-propanol-ethanol-water, the separated upper layer of which comprises the stationary phase and the bottom layer of which the mobile phase. The TPA and the phorbol isomers are obtained in exceptionally high purity by further treatment with liquid column chromatography and thin-layer chromatography.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.