Electrophoresis using alternating transverse electric fields
US4473452A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Nov 18, 1982 |
| Grant date | Sep 25, 1984 |
| Priority date | — |
| Expiry date | Nov 18, 2002 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/44773
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Disclosed are an apparatus for and a method of electrophoretically separating particles by electric fields which are transverse to each other, which alternate between respective high and low intensities out of phase with each other at a frequency related to the mass of the particles and which move the particles in an overall direction transverse to the respective directions of the fields. For separating large macromolecules, at least one of the fields preferably has an intensity gradient in a direction transverse to its own. The new arrangement makes it possible to: (1) separate particles (molecules) larger in size than those able to be separated with previously known techniques, (2) carry out separation at higher speed and at better resolution than is possible with previously known techniques, and (3) concurrently separate particles which differ greatly in mass (molecular weight).
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.