Reagent strip test for antithrombin-III
US4473639A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Sep 15, 1982 |
| Grant date | Sep 25, 1984 |
| Priority date | — |
| Expiry date | Sep 15, 2002 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/805
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Disclosed is a method and device for the quantitative determination of AT-III in mammalian blood plasma. The method involves contacting a plasma sample containing excess heparin with a 3 layered reagent strip comprising: PA1 i. a first upper layer of a bibulous material containing thrombin and a buffer; PA1 ii. a second lower layer of a bibulous material containing a fluorogenic or chromogenic substrate capable of interacting with thrombin in a time related chemical manner; and PA1 iii. a bottom layer of water impermeable material. The chemical change in the strip is determined as a function of time and this change is compared with that obtained in samples containing known amounts of AT-III.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.