Two-site immunoassays using monoclonal antibodies of different classes or subclasses and test kits for performing same
US4474892A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Feb 16, 1983 |
| Grant date | Oct 2, 1984 |
| Priority date | — |
| Expiry date | Feb 16, 2003 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/828
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Two-site immunometric assays for multideterminant antigens are described in which the antigen is reacted with an immobilized monoclonal antibody directed against one antigen determinant and a second monoclonal antibody that is directed against a distinct antigenic determinant and is of a different class or subclass than the immobilized monoclonal antibody. The second monoclonal antibody is labeled in direct versions of the assay and is reacted with a labeled antibody against it in indirect versions of the assay. The immobilizing medium and classes (subclasses) of the antibodies may be selected so as to reduce the likelihood of nonspecific binding enhance sensitivity and/or permit signal amplification.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.