Method for counting overlapping cell populations in a distribution histogram
US4475236A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Nov 12, 1981 |
| Grant date | Oct 2, 1984 |
| Priority date | — |
| Expiry date | Nov 12, 2001 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2015/1488
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A sample having a mixture of unknown stained cells and known cells having different staining characteristics is analyzed rapidly, a cell at a time, in a flow cytometry system having a sample stream dimension in the range of expected cell dimensions. The cells are illuminated with focused illumination and fluorescence is detected and related to the number of cells. The resulting histogram of the mixture sample may be analyzed by counting the cells in a controlled population below a relatively low threshold value of fluorescence intensity to form a first fraction and relating this fraction to the fraction of cells in the sample mixture below the threshold value of fluorescence intensity to determine the number of unknown cells in the sample mixture.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.