Process for the stabilization of aqueous solutions of cholesterol esterase from pseudomonas

US4476223A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Herbert Buschek · Weilheim, DE
• Helmut Schlumberger · Polling, DE
• Joachim Siedel · Bernried, DE

Key dates

• Filing date: Dec 23, 1982
• Grant date: Oct 9, 1984
• Expiry date: Dec 23, 2002

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/60
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention provides a process for the stabilization of an aqueous solution of cholesterol esterase from Pseudomonas, especially in the presence of a surface-active agent, wherein the enzyme is dissolved in a phosphate-free buffer which contains 10 to 200 mMol/liter of magnesium ions.