Detecting or quantifying substances using labelling techniques
US4478817A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Nov 14, 1978 |
| Grant date | Oct 23, 1984 |
| Priority date | — |
| Expiry date | Nov 14, 1998 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/80
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention relates to a method of detecting, analyzing, quantifying or locating a protein, antibody, antigen, hapten, hormone, metabolite, nucleic acid or steroid, in which the substance of interest is linked to a chemi-luminescent or bio-luminescent label. A luminescent reaction is then triggered by the addition of an oxidizing agent or a catalyst and the emitted light is observed in order to provide information about the substance. The invention employs a luminescent reagent which consists of antibodies labelled with a luminescent material such as luminol. The luminescent reagent can be used to quantify antigens in an immunological assay. The luminescent labelled antibodies selectively bind to the antigens and the amount of light emitted in a luminescent reaction gives an indication of the amount of antigens present. A luminescent labelled substance can also be reacted with an antibody or antigen labelled with a fluorescent material in order to carry out a homogeneous assay.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.