Method for altering red blood cell function and survival
US4478824A · kind A · utility
Inventors
Key dates
| Filing date | Aug 8, 1983 |
| Grant date | Oct 23, 1984 |
| Priority date | — |
| Expiry date | Aug 8, 2003 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61K9/5068
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Method and apparatus for creating reversible intracellular hypertonicity in red blood cells of mammals in order to introduce desired materials into the cells or achieve therapeutically desirable changes in the characteristics of intracellular hemoglobin. A packed red blood cell fraction is incubated in a solution containing a compound which readily diffuses into and out of the cells; a trans-membrane osmotic gradient is rapidly created by contacting the cells with a near-isotonic aqueous medium with or without a desired agent to be introduced, causing diffusion of water into the cells with consequent swelling thereof and permitting diffusion of said compound out of the cells; and the cells are then separated and washed to obtain a sterile fraction of substantially intact cells.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.