Tripeptide derivatives and their application in assaying enzymes
US4480030A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Sep 15, 1982 |
| Grant date | Oct 30, 1984 |
| Priority date | — |
| Expiry date | Sep 15, 2002 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/109163
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A process for quantitatively assaying factor Xa from a medium by reacting the medium with tripeptide derivatives having the formula ##STR1## wherein R.sup.5 is a chromogenic substituted amino group capable of being split off by enzymatic hydrolysis to form a colored or fluorescent product H--R.sup.5. The quantity of split product H--R.sup.5 released by the enzymatic action on the tripeptide derivative is determined photometrically, spectrophotometrically, fluorescence-spectrophotometrically, or electrochemically. The quantity of released split product H--R.sup.5 per time unit is proportional to the quantity of enzyme present in the starting material.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.