Sensitive and rapid diagnosis of viroid diseases and viruses
US4480040A · kind A · utility
56Cited by
4References
6Claims
0Family size
Assignee
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Key dates
| Filing date | Dec 3, 1981 |
| Grant date | Oct 30, 1984 |
| Priority date | — |
| Expiry date | Dec 3, 2001 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/143333
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A rapid and sensitive method for diagnosing plant viroid diseases and viruses. Plant sap is bound to a solid support and the bound sample probed with a radioactively labelled DNA that is complementary to the viroid or to the nucleic acid of the virus being diagnosed. The radioactively labelled cDNA hybridizes with that viroid or virus RNA or DNA for which it is specific. DNA-RNA and DNA-DNA hybrids are detected by autoradiographic examination of the hybridized material.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.