Method for three-volume differential determination of lymphocyte, monocyte and granulocyte populations of leukocytes
US4485175A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jan 3, 1983 |
| Grant date | Nov 27, 1984 |
| Priority date | — |
| Expiry date | Jan 3, 2003 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/107497
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method and reagent system is described for differential determination of three-populations of leukocytes (lymphocyte, monocyte and granulocyte), using an automatic counting system. The reagent system includes a blood diluent and lysing reagent. The blood diluent is an osmotically balanced aqueous solution of ingredients at a preselected pH for maintaining erythrocyte morphology, blood cell stabilizing, buffering and bacteriostatic action. The lysing reagent is a mixture of an aqueous solution of quaternary ammonium salts, which lysing reagent is added to the diluted blood under more mild conditions of concentration and at a slower rate than is current practice in order to obtain an unexpected volumetric modification of at least one of the three-populations of leukocytes, whereby volumetric differential analysis can be accomplished.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.