Creatinine specific antibody

US4485177A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Joachim Siedel · Bernried, DE
• Ulrich Neumann · Peißenberg, DE
• Joachim Ziegenhorn · Starnberg, DE
• Hans-George Batz · Tutzing, DE
• Helmut Lenz · Tutzing, DE
• Brigitte Pautz · Herrsching am Ammersee, DE
• Winfried Albert · Eberfing, DE

Key dates

• Filing date: Aug 27, 1982
• Grant date: Nov 27, 1984
• Expiry date: Aug 27, 2002

Classification

• Technology area (CPC A): Human Necessities
• CPC primary: A61K38/00
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The present invention provides a creatinine antibody and a process for the preparation thereof, wherein a conjugate of creatinine and a material suitable for antiserum formation, which are connected via an aliphatic or araliphatic carboxylic acid as bridge member, is used as immunogen for antiserum formation. The present invention is also concerned with the use of the above antibody for the immunological determination of creatinine, wherein the antibody is incubated with a creatinine-containing sample solution, reacted with a conjugate of creatinine with a hapten carrier substance, whereby one of the components, antibody and conjugate, is present in the solid phase or in dissolved form and the other component is present in dissolved form and the inhibition of the binding reaction between the antibody and the creatinine conjugate is measured. Furthermore, the present invention provides a reagent for the immunological determination of creatinine, wherein it contains the above antibody, a conjugate of creatinine with a hapten carrier substance and buffer substance.